Lesson 2.1.1a

2.1.1a Microscopy and observing cells Quiz: OCR Biology, Unit 1

20 questions

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Lesson 2.1.1a, Microscopy and observing cells: 20 multiple choice questions for the OCR Biology (H020), Unit 1: Foundations in biology, written with Revision Ninja.

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The 20 questions

  1. Which type of microscope gives high-resolution images of the internal ultrastructure of cells?

    • Scanning electron microscope
    • Dissecting microscope
    • Transmission electron microscope
    • Light microscope
  2. Which microscope produces a three-dimensional image of the surface of a specimen?

    • Light microscope
    • Transmission electron microscope
    • Scanning electron microscope
    • Phase contrast microscope
  3. Which advantage is unique to light microscopy compared to electron microscopy?

    • Greater magnification
    • Higher resolution
    • Thicker sections
    • Living specimens
  4. What is the primary function of an eyepiece graticule?

    • Providing illumination
    • Staining specimens
    • Magnifying images
    • Measuring object size
  5. What device is used to calibrate an eyepiece graticule?

    • Phase contrast lens
    • Electron beam
    • Cover slip
    • Stage micrometer
  6. What is the main purpose of differential staining in light microscopy?

    • Killing microorganisms
    • Creating a vacuum
    • Increasing magnification
    • Distinguishing cell components
  7. What formula calculates microscope magnification?

    • Actual minus image
    • Image multiplied by actual
    • Actual divided by image
    • Image divided by actual
  8. A nucleus appears 36 mm wide in a micrograph taken at x1200. What is its actual width?

    • 43.2 micrometres
    • 3 micrometres
    • 30 micrometres
    • 300 micrometres
  9. A bacterium is 2 micrometres long and appears 40 mm long in a micrograph. What is the magnification?

    • x20,000
    • x2,000
    • x80
    • x200,000
  10. A stage micrometer division is 10 micrometres. Ten eyepiece graticule divisions line up with 4 stage micrometer divisions. What is the length of one graticule division?

    • 40 micrometres
    • 0.4 micrometres
    • 4 micrometres
    • 2.5 micrometres
  11. Convert 0.5 mm to micrometres and then to nanometres.

    • 500 micrometres and 5,000 nm
    • 500 micrometres and 500,000 nm
    • 5 micrometres and 5,000 nm
    • 50 micrometres and 50,000 nm
  12. A chloroplast is 5 micrometres across and appears 75 mm across in a micrograph. What is the magnification?

    • x375
    • x150,000
    • x1,500
    • x15,000
  13. A eukaryotic cell appears 8 cm across in a micrograph at x800. What is its real diameter?

    • 1 mm
    • 6400 micrometres
    • 100 micrometres
    • 10 micrometres
  14. What happens when you enlarge an image beyond the resolution limit?

    • Magnification decreases
    • Wavelength shortens
    • Image becomes blurrier
    • Detail increases
  15. The eyepiece graticule division is calibrated as 2 micrometres at x400. What does one division represent at x1000?

    • 5 micrometres
    • 0.8 micrometres
    • 0.4 micrometres
    • 2 micrometres
  16. Why do electron microscopes require a vacuum?

    • Generate heat
    • Prevent electron scattering
    • Increase light intensity
    • Stain specimens
  17. A student views an organelle 1 micrometre across at x400. What width does it appear in the image?

    • 40 micrometres
    • 400 micrometres (0.4 mm)
    • 4 mm
    • 0.4 micrometres
  18. Which microscope type is best for viewing external surface details?

    • Transmission electron microscope
    • Light microscope
    • Scanning electron microscope
    • Laser scanning microscope
  19. A cell in a micrograph measures 60 mm across at x3000, and a mitochondrion in the same image measures 9 mm across. What is the real width of the mitochondrion?

    • 0.3 micrometres
    • 30 micrometres
    • 27 micrometres
    • 3 micrometres
  20. A graticule division is 3 micrometres at x600. A cell spans 25 graticule divisions at x1200. What is its real length?

    • 18.75 micrometres
    • 75 micrometres
    • 37.5 micrometres
    • 3 micrometres

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