Lesson 3.5.2.2

3.5.2.2 Oxidative phosphorylation and anaerobic respiration Quiz: AQA Biology, Unit 5

20 questions

Written by Revision Ninja · Last reviewed 11 October 2026

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Lesson 3.5.2.2, Oxidative phosphorylation and anaerobic respiration: 20 multiple choice questions for the AQA Biology (7402), Unit 5: Energy transfers in and between organisms, written with Revision Ninja.

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The 20 questions

  1. Where does oxidative phosphorylation take place in eukaryotic cells?

    • In the cytoplasm
    • On the inner mitochondrial membrane
    • In the mitochondrial matrix only
    • In the thylakoid membranes

  2. What is the final electron acceptor in aerobic respiration?

    • NAD
    • Pyruvate
    • Carbon dioxide
    • Oxygen

  3. What is the product formed when oxygen accepts electrons and protons at the end of the electron transfer chain?

    • Lactate
    • Carbon dioxide
    • Water
    • Ethanol

  4. What drives the production of ATP by oxidative phosphorylation?

    • Direct phosphorylation of glucose by ATP
    • Conversion of pyruvate into lactate
    • Release of carbon dioxide from the Krebs cycle
    • Movement of protons back across the inner mitochondrial membrane through ATP synthase

  5. Which theory explains how ATP is made by the movement of protons through ATP synthase, as proposed by Peter Mitchell?

    • Theory of natural selection
    • Cell theory
    • Germ theory
    • Chemiosmotic theory

  6. Why does anaerobic respiration need to regenerate NAD?

    • So that carbon dioxide can be released
    • So that oxygen can be made
    • So that acetylcoenzyme A can be formed
    • So that glycolysis can continue to produce ATP

  7. In anaerobic respiration in animal muscle, what is the final product formed from pyruvate?

    • Lactate
    • Oxygen
    • Acetylcoenzyme A
    • Ethanol

  8. In anaerobic respiration in yeast, what products are formed from pyruvate?

    • Water and oxygen
    • Glucose and water
    • Ethanol and carbon dioxide
    • Lactate only

  9. Which statement about the electron transfer chain is correct?

    • Electrons are passed down the chain, and protons are pumped across the inner mitochondrial membrane
    • Protons are pumped into the mitochondrial matrix only through the outer membrane
    • Electrons are passed to glucose to form pyruvate
    • The chain produces lactate from pyruvate

  10. Which coenzyme gives more ATP per molecule when oxidised in oxidative phosphorylation?

    • Oxidised NAD
    • Reduced FAD
    • Oxidised FAD
    • Reduced NAD

  11. Which best describes the role of ATP synthase in aerobic respiration?

    • It fixes carbon dioxide into triose phosphate
    • It breaks down glucose into pyruvate
    • It catalyses ATP synthesis as protons flow through it down their electrochemical gradient
    • It produces carbon dioxide from acetate

  12. A student states that anaerobic respiration produces more ATP per glucose than aerobic respiration. Which is the most accurate correction?

    • Anaerobic respiration produces only the net ATP from glycolysis, far less than aerobic respiration
    • Both produce exactly the same ATP per glucose
    • Anaerobic respiration produces oxygen as a useful by-product
    • Anaerobic respiration produces more ATP because it skips the Krebs cycle

  13. Which of the following is a consequence of oxidative phosphorylation depending on oxygen?

    • Lactate is produced in the mitochondrion
    • Electron transfer stops in the absence of oxygen, so ATP synthesis by this route stops
    • Glycolysis stops in the absence of oxygen
    • Pyruvate enters the nucleus

  14. Why does anaerobic respiration in muscle cause a build-up of lactate during intense exercise?

    • Lactate is made directly from glucose in the mitochondrion
    • Lactate is produced faster than it can be removed when oxygen supply is insufficient
    • Lactate is an intermediate in the Krebs cycle
    • Lactate is stored in the nucleus

  15. Calculate how many reduced NAD molecules are produced by the link reaction and Krebs cycle together, per glucose molecule.

    • 8
    • 2
    • 10
    • 6

  16. Which statement describes why the Krebs cycle depends on oxidative phosphorylation for its continued operation?

    • Reduced NAD and reduced FAD from the Krebs cycle must be reoxidised by the electron transfer chain
    • The Krebs cycle converts ethanol into lactate
    • Oxygen must be produced by the Krebs cycle for the chain to work
    • The Krebs cycle needs ATP from the electron transfer chain to fix carbon dioxide

  17. A respiring cell has its ATP synthase blocked. What is the most direct effect?

    • Lactate production in the cytoplasm increases
    • ATP synthesis by oxidative phosphorylation stops even though electron transfer continues
    • Glycolysis stops immediately
    • Carbon dioxide production in the link reaction rises

  18. Anaerobic respiration in yeast releases which gas, along with ethanol?

    • Oxygen
    • Carbon dioxide
    • Nitrogen
    • Hydrogen

  19. Where are protons accumulated during the electron transfer chain in oxidative phosphorylation?

    • In the cytoplasm, which is outside the mitochondrion
    • They are destroyed by oxygen before they reach ATP synthase
    • In the matrix only, which then move out through the outer membrane
    • In the intermembrane space, creating a gradient across the inner membrane

  20. What is the net ATP yield from anaerobic respiration of one glucose molecule in a cell, as set by glycolysis alone?

    • 38
    • 36
    • 4
    • 2

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